Journal: iScience

Article Title: Cholesterol-induced robust Ca oscillation in astrocytes required for survival and lipid droplet formation in high-cholesterol condition

doi: 10.1016/j.isci.2022.105138

Figure Lengend Snippet: Cholesterol-induced Ca oscillation requires the PLC-IP 3 pathway (A and B) Time course of Ca changes in astrocytes incubated with 2-APB (A) and thapsigargin (Tg, B) followed by cholesterol application (a) and cumulative fraction of ΔFrequency with DMSO control (b). ∗∗∗p<0.001, Kruskal-Wallis test and post hoc Bonferroni corrected Mann-Whitney U test. (C) Ca changes in U-73122 treated astrocytes (a) and ΔFrequency with a control group treated with an inactive analogue, U-73343 (b). ∗∗∗p<0.001, Kruskal-Wallis test and post hoc Bonferroni corrected Mann-Whitney U test. (D) Ca changes in cholesterol stimulated astrocytes in Ca free medium (a). ΔFrequency and amplitude are compared to the cholesterol (with Ca) group (b and c, the same data shown in Figure 2 A) and to an MCD (Ca-free) group (b). ∗p<0.05, ∗∗∗p<0.001, Kruskal-Wallis test and post hoc Bonferroni corrected Mann-Whitney U test. Number of cells(number of cultures): DMSO, 567(6); 2-APB, 359(5); Tg, 344(5); U-7312, 428(5); U-73343, 433(5); cholesterol (Cafree), 378(5); MCD (Cafree), 428(5).

Article Snippet: Thapsigargin (Tg, 33637-31, Nacalai tesque) was dissolved in DMSO at 100 μM and stored at −20°C.

Techniques: Incubation, Control, MANN-WHITNEY

Journal: iScience

Article Title: Cholesterol-induced robust Ca oscillation in astrocytes required for survival and lipid droplet formation in high-cholesterol condition

doi: 10.1016/j.isci.2022.105138

Figure Lengend Snippet:

Article Snippet: Thapsigargin (Tg, 33637-31, Nacalai tesque) was dissolved in DMSO at 100 μM and stored at −20°C.

Techniques: Recombinant, RNA Extraction, Cholesterol Assay, Plasmid Preparation, Software